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Extracellular Vesicles Isolation from Cell conditioned Media.
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Describe your sample*
Amount in ml*
Method of EV isolation *

Combination of TFF and Size Exclusion ChromatographyUltracentrifugationImmunoaffinity
In the final preparation I want:*

Total EVs together (includes exosome-like EVs and microvesicles)Only Microvesicles (EVs bigger than 150 nm)Only Exosomes -like EVs (40-150 nm EVs)
EV characterization. Isolated EVs are quantified for total protein content by BCA assay and for particle concentration and size distribution by NTA (Zetaview instrument, Particle Metrix).EV characterization. Isolated EVs are quantified for total protein content by BCA assay and for particle concentration and size distribution by NTA (Zetaview instrument, Particle Metrix).
Additional characterization:

ELISA phenotyping (screening of protein EV marker by ELISA assay)Transmission Electron Microscopy
Morphology analysisParticle count
Final form. I want that my EVs are shipped:*

Frozen (shipment in dry ice)Liquid (EVs stored at 4 degrees and shipped with ice packs)Lyophilized
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