ACQUIFER Imaging Machine - Demo User Form

To ensure the participants’ requirements and expectations for the ACQUIFER Imaging Machine Workshop at the University of Uppsala, the ACQUIFER team kindly requests all participants to fill out the following part of the demo user form.

1. Information on demo location:

Uppsala University

DanioReadout Service Facility

2. Personal Information:

Name*

First nameLast name

E-Mail*

example@example.com

Phone number

Pre-selectionNumber

Department*

Laboratory*

3. Why do you want to attend the workshop?

4. Online Seminar - Save the date:

For all participants an online seminar will be organized. In this seminar the ACQUIFER Imaging Machine will be presented. Additionally, ACQUIFER will give an overview about the range of applications the Imaging Machine can be used for.

The online Seminar will take place at the 14th of December 2023, 14:00 - 15:00.

All participants will receive an invitation before the seminar starts.

5. Online Seminar and Hands-on information:

The hands-on sessions will be held in small groups between 4 to 5 participants with 3 to 4 hours time at the Imaging Machine. Every participant is free to choose the day or days that fit best, but the ACQUIFER team will try to arrange hands-on sessions with same areas of interest. For proper planning, ACQUIFER will get in contact with all hands-on participants in person.

5.1. Choice of hands-on session:*

20th of February 2024, 9:00 to 13:0020th of February 2024, 14:00 to 18:0021st of February 2024, 9:00 to 13.0021st of February 2024, 14:00 to 18:0022nd of February 2024, 9:00 to 13.0022nd of February 2024, 14:00 to 18:00I was not able to attend the online seminar on the 14th of December, 2023. Can we meet to talk about the Imaging Machine again?

6. Information on samples:

Biological Savety: Please note that we can only handle samples that fulfil TECHlab Level S1 requirements.

6.1. Zebrafish imaging:

Used fish line

Developmental stage of zebrafish larvae (hpf)

Fixation

Living organismFixed organism

Labware reference (e.g. dish, well-plates)

Preferred orientation of zebrafish larvae for live imaging in 96-well plates:

LateralDorsalOther

Time-lapse requirements in hours or minutes:

Fluorophores used for imaging (e.g. DAPI, GFP, YFP, mCherry, TRITC):

Aim of your experiment and expectations on your hands-on session:

Further comments:

6.2. Imaging of other samples (e.g. cells, spheroids, yeast, drosophila, c. elegans)

Type of cells:

2D cell culture3D cell cultureCell suspensionOthers

Name of other sample type:

Safety Level:

NoneS1

Fixation:

Living cells / tissuesFixed cells / tissuesNo biological samples

Labware reference (e.g. dish, well-plates)

Time-lapse requirements in hours or minutes:

Fluorophores used for imaging (e.g. DAPI, GFP, YFP, mCherry, TRITC):

Aim of your experiment and expectations on your hands-on session:

Further comments:

7. Imaging data

The results of the hands-on sessions will not be shared with third parties without the consent of the participants. For potential usage of image data as supporting material (Application Notes or other marketing material) the ACQUIFER team will always ask for permission and will acknowledge the courtesy of the image data.

Thank you very much for filling out the demo user form!

For further information or if you have questions, please don´t hesitate to contact us!

bettina.trauernicht@bruker.com